The First Sign™ Oral Fluid Drug Screen Device is an immunoassay based on the principle of competitive binding. Drugs that may be present in the oral fluid specimen compete against their respective drug conjugate for binding sites on their specific antibody.
During saliva drug testing, a portion of the oral fluid specimen migrates upward by capillary action. A drug, if present in the oral fluid specimen below its cut-off concentration, will not saturate the binding sites of its specific antibody. The antibody will then react with the drug-protein conjugate and a visible coloured line will show up in the test line region of the specific drug strip. The presence of drug above the cut-off concentration in the oral fluid specimen will saturate all the binding sites of the antibody. Therefore, the colored line will not form in the test line region.
A drug non-negative oral fluid specimen will not generate a colored line in the specific test line region of the strip because of drug competition, while a drug-negative oral fluid specimen will generate a line in the test line region because of the absence of drug competition.
To serve as a procedural control, a colored line will always appear at the control line region, indicating that the proper volume of specimen has been added and membrane wicking has occurred.
- Tests for the parent THC component Delta 9 (Δ9) – not the metabolite.
- Tests for 7 drug groups as per AS/NZS 4760:2019.
- Error Free – Collection test and swab in one device.
- Less invasive than urine.
- Test anywhere – anytime.
- From collection to result in less than five minutes.
- Easy, Fast, and Accurate 1-step Saliva drug test.
- Test for 7 drugs: Marijuana THC / Cocaine COC /Amphetamine AMP / Methamphetamine METH / Opiate OPI / Benzodiazepines BZO /Oxycodone OXY.
- Allow the Saliva drug testing kit to reach room temperature (15-30°C) prior to testing. Do not place anything in the mouth including food, drink, gum, or tobacco products for at least 10 minutes prior to the collection of the oral fluid specimen.
- Bring the pouch to room temperature before opening. Remove the saliva drug test from the sealed pouch and use it as soon as possible.
- Remove the saliva drug test collection cap/sponge from the collection chamber.
- Insert the sponge end of the collection stick into the donor’s mouth. Close mouth and gently chew the sponge for saliva excretion. Soak sponge into saliva in the mouth and swab the inside of the mouth and tongue to collect oral fluid for a total of 3 minutes, until the sponge has become completely soft and fully saturated with saliva. No hard spots should be felt on the sponge when saturated.
- Remove the sponge from the mouth. With gentle pressure, place the collection stick with the saturated sponge into the collection chamber.
- Screw the collector cap clockwise to secure the cap and start the timer.
- Mark donor ID on the test device. Peel off the label to reveal the test results. Wait for the coloured lines to appear on the test strips. Read results at 10 minutes.
Test Cut-Off Levels
- Amphetamine AMP: 300ng/mL
- Benzodiazepines BZO: 200ng/mL
- Cocaine COC: 300ng/mL
- Marijuana THC: 15ng/mL
- Methamphetamine METH: 300 ng/mL
- Opiate OPI: 300ng/mL
- Oxycodone OXY: 40ng/mL
Download the Saliva drug testing Australia procedures for your convenience.
Compliant with AS/NZS 4760:2019.
All tests sold by the box – 25 tests per box.
When an order is placed prior to 12pm local EST time, below are the expected delivery times. Once an order has been dispatched we will email you the tracking number so you can track your purchase.
- Capital Cities – Next day delivery (up to 5pm next day)
- Regional Areas – Two to three days
- Remote Areas – Five days
Need your order urgently? Ask us about express courier options. 1800 187 238
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